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POLR2J regulates tumor growth in a nude mouse xenograft model. Based on the type of cells injected, the mice were assigned to three groups: NC-Vector, POLR2J knockdown (KO), and POLR2J overexpression (OE). (a) Schematic diagram of the animal experimental procedure. (b, c) Body weight curves and tumor growth curves of mice over the 18-day observation period. (d) Decay-corrected whole-body coronal <t>microPET</t> images of xenograft mice at 60 minutes after injection of 3.7 MBq of [18F]F-NOTA-PRGD2. The uptake of the quantitative tracer in the tumors and major organs is expressed as % ID/g. Tumors are indicated by white circles. (e) Final tumor weights at day 18. (f) Representative images of H&E staining, Ki-67 IHC, and POLR2J IHC in tumor tissues. The results were presented as the Means ± SD, with n = 5. ** p < 0.01 vs. the control group.
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POLR2J regulates tumor growth in a nude mouse xenograft model. Based on the type of cells injected, the mice were assigned to three groups: NC-Vector, POLR2J knockdown (KO), and POLR2J overexpression (OE). (a) Schematic diagram of the animal experimental procedure. (b, c) Body weight curves and tumor growth curves of mice over the 18-day observation period. (d) Decay-corrected whole-body coronal <t>microPET</t> images of xenograft mice at 60 minutes after injection of 3.7 MBq of [18F]F-NOTA-PRGD2. The uptake of the quantitative tracer in the tumors and major organs is expressed as % ID/g. Tumors are indicated by white circles. (e) Final tumor weights at day 18. (f) Representative images of H&E staining, Ki-67 IHC, and POLR2J IHC in tumor tissues. The results were presented as the Means ± SD, with n = 5. ** p < 0.01 vs. the control group.
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POLR2J regulates tumor growth in a nude mouse xenograft model. Based on the type of cells injected, the mice were assigned to three groups: NC-Vector, POLR2J knockdown (KO), and POLR2J overexpression (OE). (a) Schematic diagram of the animal experimental procedure. (b, c) Body weight curves and tumor growth curves of mice over the 18-day observation period. (d) Decay-corrected whole-body coronal <t>microPET</t> images of xenograft mice at 60 minutes after injection of 3.7 MBq of [18F]F-NOTA-PRGD2. The uptake of the quantitative tracer in the tumors and major organs is expressed as % ID/g. Tumors are indicated by white circles. (e) Final tumor weights at day 18. (f) Representative images of H&E staining, Ki-67 IHC, and POLR2J IHC in tumor tissues. The results were presented as the Means ± SD, with n = 5. ** p < 0.01 vs. the control group.
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POLR2J regulates tumor growth in a nude mouse xenograft model. Based on the type of cells injected, the mice were assigned to three groups: NC-Vector, POLR2J knockdown (KO), and POLR2J overexpression (OE). (a) Schematic diagram of the animal experimental procedure. (b, c) Body weight curves and tumor growth curves of mice over the 18-day observation period. (d) Decay-corrected whole-body coronal microPET images of xenograft mice at 60 minutes after injection of 3.7 MBq of [18F]F-NOTA-PRGD2. The uptake of the quantitative tracer in the tumors and major organs is expressed as % ID/g. Tumors are indicated by white circles. (e) Final tumor weights at day 18. (f) Representative images of H&E staining, Ki-67 IHC, and POLR2J IHC in tumor tissues. The results were presented as the Means ± SD, with n = 5. ** p < 0.01 vs. the control group.

Journal: Frontiers in Oncology

Article Title: POLR2J knockdown promotes ROS-induced DDR and ferroptosis by inhibiting the STAT3-GPX4 signaling axis in LUAD

doi: 10.3389/fonc.2026.1787943

Figure Lengend Snippet: POLR2J regulates tumor growth in a nude mouse xenograft model. Based on the type of cells injected, the mice were assigned to three groups: NC-Vector, POLR2J knockdown (KO), and POLR2J overexpression (OE). (a) Schematic diagram of the animal experimental procedure. (b, c) Body weight curves and tumor growth curves of mice over the 18-day observation period. (d) Decay-corrected whole-body coronal microPET images of xenograft mice at 60 minutes after injection of 3.7 MBq of [18F]F-NOTA-PRGD2. The uptake of the quantitative tracer in the tumors and major organs is expressed as % ID/g. Tumors are indicated by white circles. (e) Final tumor weights at day 18. (f) Representative images of H&E staining, Ki-67 IHC, and POLR2J IHC in tumor tissues. The results were presented as the Means ± SD, with n = 5. ** p < 0.01 vs. the control group.

Article Snippet: After 1 h, dynamic imaging was performed using an Inveon microPET scanner (Siemens Medical Solutions, Erlangen, Germany).

Techniques: Injection, Plasmid Preparation, Knockdown, Over Expression, Staining, Control